In another time and space, penicillin did not enter industrial production until more than ten years after its discovery, mainly because large-scale microbial culture techniques were not solved.
For large-scale fungal cultivation, a large amount of sterile air is added to the culture tank. If the added air contains miscellaneous bacteria, the miscellaneous bacteria multiply in the culture medium and the culture is contaminated, the production fails.
Of course, this is not a difficult problem for King Kong. He had already made two small bacterial culture jars, a twenty-liter one and a two-hundred-liter one. Sterile air is obtained using microporous ceramic filters. The bacteria in the air are blocked by the filter, and the filtered air is sterile. This is the technique used in production methods in the 21st century. Cotton is stuffed into a section of the tube and sterilized at high temperature, which can also replace this filter.
Tanaka's penicillin production has been stuck on this technical issue for a long time. Of course, he has long overcome this difficulty. He doesn't make ceramics. He uses cotton, which is stuffed tightly into a tube and sterilized to retain bacteria. But the effect is rather poor and it's easy for miscellaneous bacteria to get in.
The problem Tanaka has to overcome now is the technical difficulty of extracting penicillin from the culture medium.
The purification of antibiotics is much more difficult than the cultivation of fungi. I really don't know how the protagonists in some YY novels manage to purify them all at once.
King Kong first filters out the mycelium; Then, by extraction, remove impurities such as proteins from the filtered liquid, and vacuum dry and concentrate the resulting aqueous liquid.
Finally, he purifies by high performance liquid chromatography (through column) : he adds the concentrate to one end of a long, thin tube filled with substances such as activated carbon (he can't find silica gel, so he uses activated carbon instead), and uses a mixture of water and alcohol as the working fluid to flush the concentrate to the other end of the tube.
Because of the different molecular structures of the substance, the retention time varies as it passes through obstacles such as activated carbon; It's like having a mix of rabbits, mice, sheep, cats, etc. run a long obstacle race track together, and in the end, it's fast and slow. Rabbits, mice, etc. also have "individual differences," running fast and slow, but the same molecules have the same performance, so they come out uniform.
At the other end of the tube, pour out the liquid cup by cup one by one to see which cups contain the active ingredients. Once you have the data, you know when the liquid that comes out should be picked up and the rest can be discarded. Vacuum-dried the liquid that only received antibiotics yielded white crystalline powder.
Since Kingang was much better than Tanaka Sengo in the techniques of expanding culture and purification, Kingang's antibiotic production had surpassed that of Tanaka. Of course, when it comes to atomic bomb production, Kondo is far inferior to Tanaka, after all, they are different specialties.
Now experiments can be conducted on mice. A group of white mice were caught, their tails were cut off, the wounds were placed in a mixture of bacteria and dirty water, and wrapped in a dirty cloth. Anyway, let them get inflamed. Then inject them with various concentrations of antibiotics to see what dose is toxic and what the minimum dose is to reduce inflammation. Finally, come up with a reasonable application dose.
King Kong placed the bacterial culture workshop and the antibiotic separation and extraction workshop in two separate yards, and the workers of the two workshops were not allowed to come and go. So only a few people like King Kong and Sanfeng knew about the antibiotic production process from beginning to end. Workers only know how to operate a certain process and have no idea what they are producing.
King Kong did not know if penicillin had been patented, so he called the antibiotic he extracted "pioneer mycin".
Erfeng is causing trouble again. She cut off the tail of Little Phoenix's pet, a big mouse called Mickey. You can take Mickey's descendants for whatever you want. Little Phoenix doesn't care. But Mickey can't. He has been raised for so long that he knows Little Phoenix. Little Phoenix was crying so hard that even King Kong couldn't comfort her.
King Kong had no choice but to ask Sanfeng to bring the surgical kit, put on the magnifying glass for watch repair, and say he would sew back the tail that had been cut off. Then Little Feng stopped crying. Sanfeng's needlework was just as good as Jin's, so Jin had just started and gave instructions to Sanfeng himself.
It was intended to please the little Feng for a while, but a few days later when the bandage was removed, the tail actually grew back, only with a scar.
In the middle of March, the antibiotic was produced, and the results of animal tests were very good. But good things also need to be sold.
King Kong first went to Guangci Hospital. The vice president in charge of Guangci Hospital was a proud Englishman. In his eyes, the Englishman was the first class of the world, some white people in Europe, like the French, were the second class, like the Indians, probably the fourth or fifth class, and the Japanese were behind the Indians. The Great Chinese, of course, are behind the Japanese.
Although there were a few intelligent ones among the Great Chinese, he could believe that a certain orangutan had learned to speak, nor could he believe that the Great Chinese would invent an anti-inflammatory injection.
And it was tested on animals? Did the people of China also know about animal testing? There are differences between animals and humans, you know?
Fortunately, Dr. Ryan proved that Kim Gang's previous oral anti-inflammatory drugs were very effective. Only then did the hospital allow King Kong to be tested in the hospital on the condition that the patient themselves agreed and bear the consequences; Also, it can only be tested on Chinese patients!
Such a good medicine, King Kong still doesn't want to give it to foreigners, especially a British guy like this!
When he got to the ward, he said the new drug was officially priced at 100 yuan per tube, but it was in promotion period now, advertising, free trial!
All of a sudden, a large number of Chinese patients asked for a trial, a free premium product, not to use it for free.
King Kong selected five patients with the right condition, signed the agreement, did skin tests first, all negative, and then gave the injection.
A family member of a patient who had been in a coma with fever for two days and had been given a near-death sentence by the doctor came to ask King Kong for a test, and King Kong rushed over and gave the injection. The Briton happened to see it and sneered, what kind of panacea? Could it bring the dead back to life?
There are also patients who don't have inflammation and want medication, as if they would lose out if they didn't use it. King Kong explains that this is not a tonic and can only be used for some inflammatory diseases.
On the first day, each person gets two shots. On the second day, I also took Sanfeng to get an injection, at least for three consecutive days, to prevent the cultivation of superbugs. All six patients have improved, including this critically ill one.
I was about to return after giving the six people their morning injections when I came across five noisy people in the hall, all bloody. The most serious one had his right forearm chopped off and was held in another person's hand.
The man, who had lost his arm, refused to be rescued. Even if he was saved, he would still be disabled! Anyway, I'll cut off that Eastern ghost today, and that's enough! Another hero in twenty years!
He spoke with the same accent as AD and the others and was from Anhui.
The Anhui man who picked up his arm was also a man. You shielded me from this cut today. If you go first, I will surely kill this Oriental man, avenge you, and then go down with brother you!
King Kong went up and said, "This arm was cut off not long ago, was it?" Why not take it back?
The big man who called himself Brother Niu said, "Doctor, do you think this arm can still be reattached?"
It should be possible, though it can't be 100% guaranteed to succeed, but we should give it a try. I should have the doctor operate right away.
But the foreign doctor in the operating room says: Put your arm back? NO! NO! This is impossible! Sew up the wound right now, wrap it up, and if it doesn't get infected, you can still save your life! Do you want to reattach your severed arm? What a joke! Do you want treatment or not? If you don't treat it, get out of the way. Others are waiting to deal with it.
What attitude? Are you afraid that our Great Chinese people have no money?
King Kong placed the patient in an empty operating room next door and said to the two Anhui men, "If you agree, I'll try, but I can't guarantee success." The Anhui said, "Anyway, it's at most death. You take it."
King Kong found the anesthetic, and Sanfeng found two sterilized surgical kits. The Anhui man was given an anesthetic, the fracture was treated with hydrogen peroxide, and the operation began. Find the right direction, insert a few steel needles (the needles used for surgical sutures) into the bone to fix it, and suture the blood vessels, nerves, muscles and other tissues. Fortunately, the cut hand was flat and even the bone was still, much easier than King Kong's first limb replantation surgery.
The lighting in the operating room was good, but it still strained the eyes, and King felt tired. Sanfeng's eyes were better than that of King Kong. For the next operation, the two were switched. King Kong pointed and Sanfeng sutured. Sanfeng had seen him perform surgery before, and with skillful hands, he did it quickly and well.
While they were sewing the outer circle of skin, the foreign doctor from next door came in.
How could you do that? Do you think you can just sew it up like this? In a few days, this arm will die and rot! If more of the arm is not removed, it will rot up! The person will die! Who are you? Chinese Native Doctor! This is our hospital. We are responsible for the death!
It is only then that King Kong vaguely remembers that this amputation and subsequent surgery was first performed by our Chinese doctors in 1963, and it was also in Shanghai. Foreigners did not believe it at the time, but admitted it later.
King Kong and Anhui people said, "You don't have to take responsibility. We'll take responsibility ourselves."
Then you go out and don't stay in our hospital! All right. Anyway, the operation is done. Put on the knife ointment, bandage, splint and go.
Ryan and the vice dean came too. Ryan couldn't help King Kong this time either. How absurd!
The vice president politely said, "When the patient's hand is ready, please come to our hospital and let us see this miracle!"